going home..

One more blog from Lansing. Just talked to dad on MSN. He's now at Dubai waiting for his next flight to KL then he'll be taking the bus to Penang, arriving at 10pm while I'll be arriving at 11.15pm. Didn't meant to tell anyone the exact time I'll be back as I wanted to surprise everyone at ahmah's house. But I got too excited when I found out Dad will be arriving at the same time. So I ended up blurting out - That's great! I'll be arriving a little after you! - Oh oh..had to explain the whole thing.

Follow up of yesterday's result, confirmed that the blue colonies are false positive. They are no different from my initial plasmid. That means that when I'm back from Penang, I have to start all over again from PCR. But I think I'm going to pick more blue colonies from the ligation with more vector. Maybe there are enough for me to grow them up and mass-produce the plasmid. Oh well, will wait till I'm back before I start thinking about it. At the mean time, I am bringing home some diluted nutrient agar to isolate some soil samples from my garden just for fun.

Finished packing a while ago and realized that I managed to fit everything in one large bag and one duffel bag so now my carry on is just my laptop, which is good. Today has been crazy since I woke up at 6am. Went to lab last night at 2am to set up cultures, came home at 4am and woke up at 6am. Wasn't too happy to wake up that early but can't really sleep either as there are too many things going on in my brain. Went to lab and start running gel electrophoresis for the remaining two digestion. That was when I confirmed that my blue colonies are false positive.

Then did some culture stuff to make sure my cells will stay alive when I'm back. I intended to stay only half a day but ended up finishing only at 4pm. Came home to grab a package to be mailed home then went back to lab for prepare potassium phosphate buffer so that I can bring it home. It is required to isolate bacteria from soil. Finished around 7pm and went to Target to get some candies and chocolates. When we got home, it was almost 9pm and I still had to do laundry because I don't want to live my dirty clothes sitting around for one month. Then had dinner, talked to Hannah and CP while packing and now I'm here. I just can't wait to go home! Already thinking about going for Roti Bakar near the airport when I arrive and 'keng cheh bee hoon' the next morning at the corner of Chung Ling High School.

~excited~