Sad to say, the PCR did not work again. I have decreased my template to only 10 ng. All the annealing temperature I tried did not work either. Sigh..
I think I should try to cut the plasmid first and use the restricted plasmid as the template. Maybe it was just too complex to denature completely. Furthermore, my expected product is more than half the size of the plasmid.
oh well..will try again tomorrow with any other approach that I can think of. Time to go home to eat curry chicken!
~disappointed~
2 comments:
lol!!!! hahaha...i m laughing at myself. I only understand the last sentence in this entry... :)
awww...it's okay ler. I'm just a nerd! :P
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